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概述根據我國醫學院校的教學實際，共收錄有關實驗22個，以培養學生動手解決實際問題的能力，并加深對理論知識的理解。每個試驗均配有原理、試劑、操作步驟及試驗等相關內容，對加深學生試驗理論知識及提高英文閱讀能力有很大幫助。
目次
Chapter Ⅰ Spectrophotometry
1.1 Basic Concepts and Principle of SpectrophOtometry
1.2 Measu rement of Light Absorption
1.3 Aplications of Spectrophotometry
EXP.1 Quantitative Analysis of Protein
EXP.2 Determination of Blood Glucose
Chapter Ⅱ Electrophoresis
2.1 Basic Principles of Electrophoresis
2.2 Impact Factors of Electrophoresis
2.3 Detection of Components after Electrophoretic Separation
2.4 Special Electropho retic Techniques and Applications
EXP.3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
EXP.4 Separation of Serum Lipoproteins by Agarose Gel Electrophoresis
EXP.5 Proteins Separation by SDS—Polyacrylamide Gel Electrophoresis
Chapter Ⅲ Chromatography
3.1 Fundamental Principles of Chromatography
3.2 Com monly Used Chromatog raphiC Techniques
EXP.6 Detecting Transamination of Amino Acids by Paper Chromatography
EXP.7 Separation of Mixed Amino Acids by Cation Exchange Chromatography
EXP.8 Separation of Hemoglobin and Dinitrophenyl(DNP)-glutamate by Gel Filtration Chromatography
Chapter Ⅳ Enzyme Analysis
4.1 The Activity of Enzyme
4.2 Enzyme kinetics(Factors affecting reaction velocity)
4.3 Enzvmarie analysis
EXP.9 Assay the Activity of Alanine Aminotransferase(ALT)in Serum (Mohun’S Method)
EXP.10 Lactate Dehydrogenase(LDH)Analysis
EXP.11 Effect of Substrate Concentration on Enzyme Activity.Determining Km Value for Alkaline Phosphatase
EXP.12 Coupled enzymatic reaction assay(EnzymaticEndpoint Method)-Determination of Total Cholesterol，Triglycerides and
Glucose in Serum
Chapter Ⅴ Isolation and Purification of Protein
5.1 Methods and Basic Principles of Protein Pu rification
5.2 Procedure of Protein Preparation
5.3 Crystallization，Concentration，Drying and Storage of Protein
5.4 Identification and Analysis of Protein
EXP.13 Isolation and Identification of Serum IgG
EXP.14 Expression.Purification and Identification of Glutathione S.Transferase Fusion Protein
Chapter Ⅵ Isolation.Purification and Identification of Nucleic Acids
6.1 Isolation and Purification of Nucleic Acids
6.2 Identification and Analysis of Nucleic Acids
EXP.15 Isolation of Eukaryotic Genomic DNA by Proteinase K.Phenolor NaI Method
EXP.16 SDS.Alkaline Lysis of Plasmid DNA
EXP.17 Isolation of Total RNA by TRIzol Reagent
……
Chapter Ⅶ Genetic Engineering
English-Chinese Index
1.1 Basic Concepts and Principle of SpectrophOtometry
1.2 Measu rement of Light Absorption
1.3 Aplications of Spectrophotometry
EXP.1 Quantitative Analysis of Protein
EXP.2 Determination of Blood Glucose
Chapter Ⅱ Electrophoresis
2.1 Basic Principles of Electrophoresis
2.2 Impact Factors of Electrophoresis
2.3 Detection of Components after Electrophoretic Separation
2.4 Special Electropho retic Techniques and Applications
EXP.3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
EXP.4 Separation of Serum Lipoproteins by Agarose Gel Electrophoresis
EXP.5 Proteins Separation by SDS—Polyacrylamide Gel Electrophoresis
Chapter Ⅲ Chromatography
3.1 Fundamental Principles of Chromatography
3.2 Com monly Used Chromatog raphiC Techniques
EXP.6 Detecting Transamination of Amino Acids by Paper Chromatography
EXP.7 Separation of Mixed Amino Acids by Cation Exchange Chromatography
EXP.8 Separation of Hemoglobin and Dinitrophenyl(DNP)-glutamate by Gel Filtration Chromatography
Chapter Ⅳ Enzyme Analysis
4.1 The Activity of Enzyme
4.2 Enzyme kinetics(Factors affecting reaction velocity)
4.3 Enzvmarie analysis
EXP.9 Assay the Activity of Alanine Aminotransferase(ALT)in Serum (Mohun’S Method)
EXP.10 Lactate Dehydrogenase(LDH)Analysis
EXP.11 Effect of Substrate Concentration on Enzyme Activity.Determining Km Value for Alkaline Phosphatase
EXP.12 Coupled enzymatic reaction assay(EnzymaticEndpoint Method)-Determination of Total Cholesterol，Triglycerides and
Glucose in Serum
Chapter Ⅴ Isolation and Purification of Protein
5.1 Methods and Basic Principles of Protein Pu rification
5.2 Procedure of Protein Preparation
5.3 Crystallization，Concentration，Drying and Storage of Protein
5.4 Identification and Analysis of Protein
EXP.13 Isolation and Identification of Serum IgG
EXP.14 Expression.Purification and Identification of Glutathione S.Transferase Fusion Protein
Chapter Ⅵ Isolation.Purification and Identification of Nucleic Acids
6.1 Isolation and Purification of Nucleic Acids
6.2 Identification and Analysis of Nucleic Acids
EXP.15 Isolation of Eukaryotic Genomic DNA by Proteinase K.Phenolor NaI Method
EXP.16 SDS.Alkaline Lysis of Plasmid DNA
EXP.17 Isolation of Total RNA by TRIzol Reagent
……
Chapter Ⅶ Genetic Engineering
English-Chinese Index
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