從基因到基因組-DNA技術概念和應用(簡體書)

基因技術、遺傳工程、重組DNA和基因克隆等技術的快速發展，把分子生物學推到了生命科學的前沿。本書對分子生物學核心技術的原理及其使用進行了簡明扼要的闡釋。以對分子生物學的基本概念的簡短介紹開篇，然后介紹核心的分子生物學方法及其整合運用，從單個基因的克隆與研究到整個基因組的測序，以及基因組信息的分析。最后，本書還介紹了這些技術在生物技術、醫藥和農業以及整個生命科學研究中的應用。 本書內容涵蓋了分子生物學基礎、基因克隆、核酸的純化與分離、DNA的剪切與連接、載體、基因組文庫和cDNA文庫、聚合酶鏈反應、DNA測序、序列數據分析、基因變異分析、基因表達分析、基因功能分析、醫藥應用、轉基因等等方面。 本書適于生物化學、分子生物學、遺傳學、細胞生物學、生物技術、生物工程等相關學科及研究領域的本科生、研究生及教學科研人員參考使用。

Preface
1 Introduction
2 Basic Molecular Biology
　2.1 Nucleic Acid Structure
　2.2 Gene Structure and Organization
　2.3 Information Flow:Gene Expression
3 How to Clone a Gene
　3.1 What is Cloning?
　3.2 Wverview of the Procedures
　3.3 Gene Libraries
　3.4 Hybridization
　3.5 Polymerase Chain Reaction
4 Purification and Separation of Nucleic Acids
　4.1 Extraction and Purification of Nucleic Acids
　4.2 Detection and Quantitation of Nucleic Acids
　4.3 Gel Electrophoresis
5 Cutting and Joining DNA
　5.1 Restriction Endonucleases
　5.2 Ligation
　5.3 Alkaline Digests
　5.4 Double Digests
　5.5 Modification of Restriction Fragment Ends
　5.6 Other Ways of Joining DNA Molecules
　5.7 Summary
6 Vectors
　6.1 Plasmid Vectors
　6.2 Vectors Based on the Lambda Bacteriophage
　6.3 Cosmids
　6.4 M13 Vectors
　6.5 Expression Vectors
　6.6 Vectors for Cloning and Expression in Eukaryotic Cells
　6.7 Supervectors:YACs and BACs
　6.8 Summary
7 Genomic and cDNA Libraries
　7.1 Genomic Libraries
　7.2 Growing and Storing Libraries
　7.3 cDNA Libraries
　7.4 Random,Arrayed and Ordered Libraries
8 Finding the Right Clone
　8.1 Screening Libraries with Gene Probes
　8.2 Screening Expression Libraries with Antibodies
　8.3 Rescreening
　8.4 Subcloning
　8.5 Characterization of Plasmid Clones
9 Polymerase Chain Reaction(PCR)
　9.1 The PCR Reaction
　9.2 PCR in Practice
　9.3 Cloning PCR Products
　9.4 Long-range PCR
　9.5 Reverse-transcription PCR
　9.6 Rapid Amplification of cDNA Ends(RACE)
　9.7 Applications of PCR
10 DNA Sequencing
　10.1 Principles of DNA Sequencing
　10.2 Automated Sequencing
　10.3 Extending the Sequence
　10.4 Shotgun Sequencing:Contig Assembly
　10.5 Genome Sequencing
11 Analysis of Sequence Data
　11.1 Analysis and Annotation
　11.2 Databanks
　11.3 Sequence Comparisons
12 Analysis of Genetic Variation
　12.1 Nature of Genetic Variation
　12.2 Methods for Studying Variation
13 Analysis of Gene Expression
　13.1 Analysing Transcription
　13.2 Comparing Transcriptomes
　13.3 Methods for Studying the Promoter
　13.4 Translational Analysis
14 Analysis of Gene Function
　14.1 Relating Genes and Functions
　14.2 Genetic Maps
　14.3 Relating Genetic and Physical Maps
　14.4 Linkage Analysis
　14.5 Transposon Mutagenesis
　14.6 Allelic Replacement and Gene Knock-outs
　14.7 Complementation
　14.8 Studying Gene Function through Protein Interactions
15 Manipulating Gene Expression
　15.1 Factors Affecting Expression of Clonde Genes
　15.2 Expression of Cloned Genes in Bacteria
　15.3 Expression in Eukaryotic Host Cells
　15.4 Adding Tags and Signals
　15.5 In vitro Mutagenesis
16 Medical Applications,Present and Future
　16.1 Vaccines
　16.2 Detection and Identification of Pathogens
　16.3 Human Genetic Diseases
17 Transgenics
　17.1 Transgenesis and Cloning
　17.2 Animal Transgenesis and its Applications
　17.3 Transgenic Plants and their Applications
　17.4 Summary
Bibliography
Glossary
Index