滿額折
Cloning of oil degradation genes
商品資訊
相關商品
商品簡介
商品簡介
A novel bacterium was engineered with an effective expression system to improve its ability to degrade oil in contaminated environments. Enrichment culture techniques were used with crude oil as the sole carbon source to isolate microorganisms. Seventeen bacterial isolates were identified using biochemical tests and 16S rRNA gene sequencing. Identified genera included Acinetobacter, Pseudomonas, Aeromonas, Micrococcus, Achromobacter, Enterobacter, Klebsiella, Staphylococcus, Bacillus, Exiguobacterium, Brevibacillus, and Stenotrophomonas. A specific primer was designed to amplify the alkB gene, confirming its presence in Pseudomonas aeruginosa. Plasmid curing confirmed the gene's presence on the chromosome. The alkB gene was cloned into a plasmid vector, transformed into E. coli, and confirmed via PCR. Expression of the gene was verified, and its contribution to biodegradation efficiency was tested using n-hexadecane as a substrate. Biodegradation efficiency increased from 32.63% to 77.42% after 72 hours, indicating a 44.8% improvement. Gene amplification and cloning strategies were simulated using SnapGene software. This study is the first of its kind in Iraq.
您曾經瀏覽過的商品
購物須知
外文書商品之書封，為出版社提供之樣本。實際出貨商品，以出版社所提供之現有版本為主。部份書籍，因出版社供應狀況特殊，匯率將依實際狀況做調整。
無庫存之商品，在您完成訂單程序之後，將以空運的方式為你下單調貨。為了縮短等待的時間，建議您將外文書與其他商品分開下單，以獲得最快的取貨速度，平均調貨時間為1~2個月。
為了保護您的權益，「三民網路書店」提供會員七日商品鑑賞期(收到商品為起始日)。
若要辦理退貨，請在商品鑑賞期內寄回，且商品必須是全新狀態與完整包裝(商品、附件、發票、隨貨贈品等)否則恕不接受退貨。