Plant Lipids: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

Part I Basic Methods of Lipid Isolation and Analysis

1 Lipid Isolation from Plants
Jesse D. Bengtsson, James G. Wallis, and John Browse

2. Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield
Samira Mahboub, Zachery D. Shomo, R. Maxwell Regester, Mahaa Albusharif, and Rebecca L. Roston

3. Thin-Layer Chromatography
Georg H闤zl and Peter D顤mann

4. Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry
Mathias Brands, Philipp Gutbrod, and Peter D顤mann

5. 14C-Tracing of Lipid Metabolism
Hari Kiran Kotapati and Philip D. Bates

6. Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes
Masako Iwai, Shiori Shibata, Hiroyuki Ohta, and Koichiro Awai

Part II Mass Spectrometry and NMR Analysis

7. Direct Infusion Mass Spectrometry for Complex Lipid Analysis
Katharina Gutbrod, Helga Peisker, and Peter D顤mann

8. Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry
Pamela Tamura, Carl Fruehan, David K. Johnson, Paul Hinkes, Todd D. Williams, and Ruth Welti

9. Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS
Cornelia Herrfurth, Yi-Tse Liu, and Ivo Feussner

10. Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism
Rebecca E. Cahoon, Ariadna Gonzalez Solis, Jonathan E. Markham, and Edgar B. Cahoon

11. Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS
Richard Broughton and Fr嶮廨ic Beaudoin

12. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae
Richard P. Haslam and Tony R. Larson

13. Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS
Lauren M. Jenkins, Jeong-Won Nam, Bradley S. Evans, and Doug K. Allen

14. Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy
Wiebke Knaack, Georg H闤zl, and Nicolas Gisch

Part III Lipid Isolation and Analysis from Plant Tissues Cell Compartments and Organelles

15. Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds
Johanna Baales, Viktoria V. Zeisler-Diehl, and Lukas Schreiber

16. Isolation of Lipid Droplets for Protein and Lipid Analysis
Patrick J. Horn, Kent D. Chapman, and Till Ischebeck

17. Isolation of Plastoglobules for Lipid Analyses
Denis Coulon and Claire Br嶭幨in

18. Isolation of Mitochondria for Lipid Analysis
S嶵astien Leterme, Morgane Michaud, and Juliette Jouhet

19. Collection and Analysis of Phloem Lipids
Susanne Hoffmann-Benning

Part IV Lipid Signaling, Lipid-Protein Interactions, and Imaging

20. Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC
Debabrata Laha, Mar璱ia Kamleitner, Philipp Johnen, and Gabriel Schaaf

21. Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography
Larissa Launhardt, Monique Matzner, Mareike Heilmann, and Ingo Heilmann

22. Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays
Guido Ufer, Peter D顤mann, and Dorothea Bartels

23. Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC)
Ze-Hua Guo and Mee-Len Chye

24. In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI)
Drew Sturtevant, Mina Aziz, Trevor B. Romsdahl, Chase D. Co